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endothelial cells  (Vector Laboratories)


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    Structured Review

    Vector Laboratories endothelial cells
    Endothelial Cells, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/L-1100-5/Unconjugated+Griffonia+(Bandeiraea)+Simplicifolia+Lectin+I+(GSL+I%2C+BSL+I)/pm42020738-484-10-13
    Average 95 stars, based on 433 article reviews
    endothelial cells - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Single cell spatial profiling of the matrisome identifies region-specific adhesion and signaling networks in glioblastoma.
    Article Snippet: Following washes with PBS-Tween20, the sections were incubated with the following secondary antibodies at RT for 1 hour: Alexa Flour® 594 donkey anti-chicken (1:500, cat#703-585-155, Jackson ImmunoResearch), Alexa Flour® 488 donkey anti-rabbit (1:500, cat#711-545-152, Jackson ImmunoResearch) and Alexa Fluor® 647 donkey anti-goat (1:500, cat#705-605-147, Jackson ImmunoResearch). .. Secondary antibodies used for immunohistochemistry include horse anti-goat IgG (H+L), biotinylated (1:500, cat#BA-9500, Vector Laboratories Inc.) and horse anti-rabbit IgG (H+L), biotinylated (1:500, cat#BA-1100, Vector Laboratories Inc.). .. Image acquisition and analysis Immunofluorescence images were acquired using Olympus FLUOVIEW FV3000 confocal laser scanning microscope 34.

    Article Title: Single cell spatial profiling of the matrisome identifies region-specific adhesion and signaling networks in glioblastoma
    Article Snippet: Following washes with PBS-Tween20, the sections were incubated with the following secondary antibodies at RT for 1 hour: Alexa Flour® 594 donkey anti-chicken (1:500, cat#703-585-155, Jackson ImmunoResearch), Alexa Flour® 488 donkey anti-rabbit (1:500, cat#711-545-152, Jackson ImmunoResearch) and Alexa Fluor® 647 donkey anti-goat (1:500, cat#705-605-147, Jackson ImmunoResearch). .. Secondary antibodies used for immunohistochemistry include horse anti-goat IgG (H + L), biotinylated (1:500, cat#BA-9500, Vector Laboratories Inc.) and horse anti-rabbit IgG (H + L), biotinylated (1:500, cat#BA-1100, Vector Laboratories Inc.). .. Immunofluorescence images were acquired using Olympus FLUOVIEW FV3000 confocal laser scanning microscope .

    Incubation:

    Article Title: Role of inflammatory response in benzo[a]pyrene-induced noradrenergic axon degeneration in mouse brain.
    Article Snippet: .. Subsequently, they were incubated with a biotinylated secondary horse anti-rabbit IgG antibody (H+L), R.T.U (BP-1100–50, Vector Laboratories, Inc., Newark Burlingame, CA) for anti-NAT and anti-Iba1 microglia for 1 hr at room temperature. .. Later, the sections were washed three times with TBST for 5 min each, followed by incubation with VECTASTAIN Elite ABC reagent, Peroxidase (Vector Laboratories) for 45 min to increase their adherence to the biotinylated target.

    Article Title: The human olfactory bulb in Huntington's disease: sex-specific differences in volume and gliosis.
    Article Snippet: Astroglia were identified using the anti-GFAP rabbit polyclonal antibody DAKO (1,10,000 in PBS + 0.1% TX-100 + 10% normal horse serum) and microglial population (in different states) were identified using the anti-Iba1 rabbit polyclonal antibody Wako (12,000 in PBS + 0.1% TX-100). .. Next, the sections were incubated for 2 h in secondary antibody (biotinylated horse anti-rabbit IgG (H + L) Vector Laboratories, 1:200 in PBS + 0.1% TX-100 + 10% normal horse serum). .. Afterward, the avidin-biotin complex (ABC standard, Vector Laboratories, containing 0.3% TX-100) was applied to the sections for 90 min, after which the sections were reacted with 3,3′-diaminobenzidine (Sigma–Aldrich, Saint Louis, MI, USA) and 0.1% H2O2.

    Article Title: Spatially patterned cytoskeletal organization shapes astrocyte branch complexity
    Article Snippet: .. The day before immunopanning, 15 cm Petri dishes were coated by adding 60 μL secondary antibody, or 100 μg BSL-1 lectin (Vector Laboratories, L-1100-5), to 20 mL of 50 mM Tris-HCl (pH 9.5), ensuring the entire dish surface was covered, and incubated overnight at 4 °C. .. The day before immunopanning, 15 cm Petri dishes were coated by adding 60 μL secondary antibody, or 100 μg BSL-1 lectin (Vector Laboratories, L-1100-5), to 20 mL of 50 mM Tris-HCl (pH 9.5), ensuring the entire dish surface was covered, and incubated overnight at 4 °C.

    Suspension:

    Article Title: Astrocytes connect specific brain regions through plastic networks.
    Article Snippet: Tissue was then triturated using a 5 ml serological pipette to generate a single cell suspension, resuspended in Dulbecco’s PBS (VWR, SH30264.FS) with BSA (BSA, Sigma-Aldrich, A4161) and DNase (Worthington Biochemical, LS002007), and filtered using a 20 μm nitex filter. .. The suspension was negatively panned for non-specific secondary antibody binding, endothelial cells (BSL-1, Vector Labs L-1100), microglia (CD45, BD Pharmingen, 553076) and oligodendrocyte lineage cells (O4 hybridoma, generated in house), and positively panned for astrocytes (ITGB5, Thermo Fisher Scientific, 14-0497-82). .. Astrocytes were removed from the positive panning plate using TrypLE (Thermo Fisher Scientific, 12-605-010) and plated at 10,000 cells per well in an eight-well chamber slide precoated with poly-l-lysine (Ibidi 80804).

    Binding Assay:

    Article Title: Astrocytes connect specific brain regions through plastic networks.
    Article Snippet: Tissue was then triturated using a 5 ml serological pipette to generate a single cell suspension, resuspended in Dulbecco’s PBS (VWR, SH30264.FS) with BSA (BSA, Sigma-Aldrich, A4161) and DNase (Worthington Biochemical, LS002007), and filtered using a 20 μm nitex filter. .. The suspension was negatively panned for non-specific secondary antibody binding, endothelial cells (BSL-1, Vector Labs L-1100), microglia (CD45, BD Pharmingen, 553076) and oligodendrocyte lineage cells (O4 hybridoma, generated in house), and positively panned for astrocytes (ITGB5, Thermo Fisher Scientific, 14-0497-82). .. Astrocytes were removed from the positive panning plate using TrypLE (Thermo Fisher Scientific, 12-605-010) and plated at 10,000 cells per well in an eight-well chamber slide precoated with poly-l-lysine (Ibidi 80804).

    Generated:

    Article Title: Astrocytes connect specific brain regions through plastic networks.
    Article Snippet: Tissue was then triturated using a 5 ml serological pipette to generate a single cell suspension, resuspended in Dulbecco’s PBS (VWR, SH30264.FS) with BSA (BSA, Sigma-Aldrich, A4161) and DNase (Worthington Biochemical, LS002007), and filtered using a 20 μm nitex filter. .. The suspension was negatively panned for non-specific secondary antibody binding, endothelial cells (BSL-1, Vector Labs L-1100), microglia (CD45, BD Pharmingen, 553076) and oligodendrocyte lineage cells (O4 hybridoma, generated in house), and positively panned for astrocytes (ITGB5, Thermo Fisher Scientific, 14-0497-82). .. Astrocytes were removed from the positive panning plate using TrypLE (Thermo Fisher Scientific, 12-605-010) and plated at 10,000 cells per well in an eight-well chamber slide precoated with poly-l-lysine (Ibidi 80804).



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